The objectives of this study were to compare clinical retention of Local Made Sealant (MTEC clear) and imported sealant (Delton clear) and to examine dental caries after sealant loss. The sealant was applied randomly on 150 first permanent molars using a half-mouth design. The retention was investigated as total retention, partial loss, and total loss. The retention of two sealants was compared using Chi-square test. When considering the sealant retention on occlusal surface, there, there was significant difference at 12, 24, 30 and 36 months, respectively (p=.027, .003, .000, .0030). The retention of sealant on occlusal surface and buccal pit and groove of lower molars or lingual groove of upper molars were significantly different at 12, 18, 24, 30 and 36 months, respectively (p=.020, .006, .027, .000, .003). The retention rate of Delton was higher than local made sealant. The carious lesion was detected on occlusal surface of teeth previously sealed with MTEC and Delton at 24 and 30 months, respectively. Buccal or lingual pit caries of the two sealants were found at 24 months.The objectives of this study were to compare clinical retention of Local Made Sealant (MTEC clear) and imported sealant (Delton clear) and to examine dental caries after sealant loss. The sealant was applied randomly on 150 first permanent molars using a half-mouth design. The retention was investigated as total retention, partial loss, and total loss. The retention of two sealants was compared using Chi-square test. When considering the sealant retention on occlusal surface, there, there was significant difference at 12, 24, 30 and 36 months, respectively (p=.027, .003, .000, .0030). The retention of sealant on occlusal surface and buccal pit and groove of lower molars or lingual groove of upper molars were significantly different at 12, 18, 24, 30 and 36 months, respectively (p=.020, .006, .027, .000, .003). The retention rate of Delton was higher than local made sealant. The carious lesion was detected on occlusal surface of teeth previously sealed with MTEC and Delton at 24 and 30 months, respectively. Buccal or lingual pit caries of the two sealants were found at 24 months.