JOURNAL OF THE DENTAL ASSOCIATION OF THAILAND

  • Issue 5 volume 53
  • Issue 4 volume 53
  • Issue 3 volume 53
  • Issue 2 volume 53
  • Issue 1 volume 53
1
Page : 287-301

Title : Auricular reconstruction with extraoral implants and magnetic attachment: a report of 7 cases

Author(s) : Panarat Kodkeaw, Piyawat Phankosol, Niyom Thamronganansakul, Kussalee Wilairat, Somchai Sessirisombat

Keyword(s) : auricular prosthesis,extraoral implant,magnetic attachment

Manuscript Type : Original Article (บทวิทยาการ)

Page : 287-301

Auricular defects may produce functional and psychological impairments that adversely affect a patient quality of life. although surgical reconstruction is the treatment of choice, the auricular prosthesis should be considered when surgical reconstruction is not possible. This case report described 7 volunteer patients treated with implant retained auricular prostheses using magnetic attachments at the Maxillofacial Prosthetic Rehabilitation Clinic, Dental Hospital, Chulalongkorn University, since November 2001 to October 2002. Among 7 Patients, there are 3 patients with congenital defect and 4 patients with traffic accident defect. All of the patients were interviewed for personal information and attitude before and after treatment. Two Ankylos extraoral implants were placed in to temporal and mastoid bone for retaining each auricular prosthesis. After placing attaching of implant abutment, patients were instructed and motivated to clean peri-implant soft tissue and implant abutment gently and carefully. Patients were evaluated for cleansing efficiency and following up until hygienic performing was acceptable. After handing the prosthesis, patients were reinstructed for prosthesis and implant maintenance. The result from the post operative questionnaire showed that all patients appreciated the treatment procedure and were satisfied with the results. All implants survived without mobility or tissue reaction that need surgical correction. Patients could take care of implant abutments, surrounding tissue and prosthesis satisfactority.
2
Page : 302-312

Title : Effect of resin composite reinforcement in post and core restoration of teeth with flared root canal

Author(s) : Laddawan Sumpansirikul, Prarom Salimee

Keyword(s) : flared root canal,fracture resistance,post and core restoration,resin reinforcement

Manuscript Type : Original Article (บทวิทยาการ)

Page : 302-312

This study aimed to investigate the effect of reinforcement with resin composite in endodontically treated teeth with flared root canal by comparing fracture resistance and fracture mode of restoration. 3 groups (n=10, each) of post and core restoration made from base metal alloy on extracted human maxillary central incisors were performed, normal root canal restored with cast post and core (group 1), flared root canal restored with cast post and core (group 2), resin composite reinforced at flared root canal before restored with cast post and core (group 3). Posts were cemented in root canal using resin cement. The fracture resistance when loaded on lingual surface of core in group 1, group 2 and group 3 were 1,126.9±75.5 N, 489.4±48.7 N, and 639.9±48.3 N, respectively. One way ANOVA and Scheffe statistical analysis were performed (α=0.01). The analysis showed that reinforcement with resin composite in flared root canal could significantly increase the fracture resistance when restored with cast post and core and resulted in shallower depth of root fracture. However, the fracture resistance of this group is significantly lower than group with normal root canal.
3
Page : 313-322

Title : Effect of elastic recovery time on dimensional stability of polysulfide impression material

Author(s) : Parintorn Hariraksapitak, Boonlert Kukiattrakoon

Keyword(s) : dimensional stability elastic recovery time, polysulfide

Manuscript Type : Original Article (บทวิทยาการ)

Page : 313-322

The objective of this study was to examine the effects of elastic of elastic recovery duration and the number in repeatedly pouring stone models on the three dimensional stability of light and regular body polysulfide impression materials (Permlastic®, Kerr Corporation, MI, USA). Light and regular body impressions of a rectangular master stainless steel die (10x10x15 mm³) was made with special tray. The stone die were made with type IV dental stone (Velmix®, Kerr Corporation, MI, USA) immediately or at 30 and 60 minutes after complete polymerization (n=10/each). Pouring procedure of each die was indicated to follow the durational classification of each main group as well. The specimens were prepared repeatedly until the first, second and third stone die comprised 10 pieces of each, totally 90 pieces. Triplicated die was made from each impression and each pouring time was 30 and 60 minutes apart. After the complete setting, the dimension of stone dies were measured and then calculated for the volume in cubic millimeter. Data were statistically analyzed with two-way ANOVA and Tukey post-hoc test at α=0.05. The result showed that, volumetrically, all stone die were significantly bigger than the master stainless steel die (1526.02 mm³) at p≤0.05. Considering the effect of elastic recovery duration in the light body polysulfide impression material group, the immediate pouring stone die (1543.64±6.88 mm³) was volumtrically smaller than the 30 minute-delayed (1547.41±7.21 mm³) and 60 minute-delayed die (1547.23±7.24 mm³) significantly at p≤0.05, while the sizes of two latter groups were not significantly different (p>0.05). The result showed no significant difference of the sizes of the stone dies that were poured immediately (1585.36±14.14 mm³) and 30 min. (1591±14.69 mm³). However, the stone dies that were poured at 60 min. (1602.46±14.25 mm³) were significant greater than those at immediately and 30 min. (p≤0.05). The study of the effect of repeated use of the light and regular body impressions showed similar results. Considering the effect of the number in repeated pouring stone models, the results between the light and regular body polysulfide impression material group, that is, the first stone die (1539.70±5.31 mm³ and 1581.86±12.77 mm³) was significantly smaller than the second (1546.14±5.09 mm³ and 1594.36±14.05 mm³) and the third stone die (1552.45±4.81 mm³ and 1603.59±12.86 mm³) consecutively (p≤0.05). this study suggested that the three dimensional stability of polysulfide impression material would be less satisfactory for the longer providing elastic recovery time and/or the increasing of number in repeated pouring with dental stone. Generally, fabricating dental stone die with polysulfide impression material should be done immediately once it completely set to achieve the best accuracy of the stone dies.
4
Page : 323-339

Title : Comparative study of forces generated by orthodontic leveling archwires tired at different levels of tooth irregularity

Author(s) : Vachara Phetcharakupt, Chintana Sirichompun

Keyword(s) : forces, leveling archwire, unloaded forces

Manuscript Type : Original Article (บทวิทยาการ)

Page : 323-339

The purpose of this study were to compare the generated forces and the unloaded forces generated by various types of orthodontic leveling archwires, as well as cross-section areas (CA), at each level of tooth irregularity (TI) (0.25-2.0 mm.); and to comparatively rank them by focussing on 7 types of leveling archwires, namely, Respond, Tripleflex, D-rect, Ni-Ti, each archwire was performed in the lower arch epoxy resin dentoform by using LLOYD universal testing machine LR 10 K. The forces were analytically compared by an Independent-Sample T-test and a One-way ANOVA at p=0.05. In the study of the forces generated by the leveling archwires at 2.0 mm. TI, there was significant difference (p<0.005) among various types of wires and those of different cross-sectional areas. However there was none for the followings: D-rect .017x.025 and .018x.025 inch² (p=1.0), Ni-Ti .014 inch and Triple flex .015 inch (p=1.0), Ni-Ti .017 x.025 and .018x.025 inch² (p=0.717), Turbo .017x.025 inch² Tripleflex .0175 inch (p=0.539), F-9 .016x.022 inch² and D-rect .016x.022 inch² (p=1.0). As for the study of the forces and deactivated forces generated by the same types of archwires with the same CA no significant difference was found as follows at Ti 1.0 mm. Respond .0155 inch from 2.0 and 1.5 mm. (p=0.074): Ni-Ti .017x.022 with and .018x.025 inch² from Ti 2.0 and 1.5 mm. (p=0.074): Ni-Ti .016x0.22 and .018x.025 inch² from TI 2.0 and 1.5 mm. (0.017, 0.183 respectively) and also at TI=0.25 and 0.5 mm. Ni-Ti of different CA (including Turbo) at different TI. However, the study showed that the deactivation from TI=1.5 mm. To TI=0.25 mm. can not be done by TMA .016x.22 and .017x.025 inch². Moreover, the deactivation from TI=2 mm. to TI=0.25 mm. can not be done by various types of archwires and CA; except every type of Ni-Ti and Turbo. Tables and figure of forces and deactivation forces of archwires from this study will be beneficial for orthodontists in wire selection as well as during progression.
5
Page : 340-348

Title : The relationship between maxillary incisors and other cephalometric measurments in class II division 2 malocclusion.

Author(s) : Korapin Mahatumarat, Piyarat Apivatanagul

Keyword(s) : cephalometric measurement,class II division 2 malocclusion,incisal retroclination

Manuscript Type : Original Article (บทวิทยาการ)

Page : 340-348

The objective of this study to find the relationship between the position and the inclination of the maxillary incisors and other cephalometric measurements in a group of samples including 35 Thai patients who have class II division 2 malocclusion. The average age was 19 years. All of the samples finished the pubertal growth spurt. Lateral cephalometric radiographs of each sample was traced and measured. Means of the cephalometric measurements were used to analyze the correlation coefficient between the position and the inclination of the maxillary incisors and other cephalometric measurements. The findings were 1. Most of the correlation coefficients between the measurements of the maxillary incisors (position and inclination) and the relationship of maxilla and mandible were mild to moderate and were statistically significant, 2. Almost all of the correlation coefficients between the measurements of the maxillary incisors and the position and the size of maxilla, mandible and cranial base were mild to very mild and were not statistically significant, 3. Most of the correlation coefficients between the measurements of the maxillary incisors and the measurements of the mandibular incisors (position and inclination) were mild to marked and were statistically significant. The results of this study could be applied to the treatment planning of this kind of malocclusion.
6
Page : 349-356

Title : A Retrospective Study of 486 Cases of Ameloblastomas

Author(s) : Kittipong Dhanuthai, Soranun Chantarangsu, Ekarat Phattarataratip, Somsri Rojanawatsirivej

Keyword(s) : ameloblastoma,retrospective,Thai

Manuscript Type : Original Article (บทวิทยาการ)

Page : 349-356

Ameloblastoma is the most common odontogenic tumor of the jaw. Of the total of 11,599 oral and maxillofacial biopsies from the files of the Department of Oral Pathology, Faculty of Dentistry, Chulalongkorn University during 1982-2002, 486 cases (4.19%) were diagnosed as ameloblastoma. Data with respect to the patients age, gender, tumor location, radiographic finding as well as histologic finding were collected and analyzed. The results showed that ameloblastomas were found in patients from 4-83 years of age (mean 34.37 years). Two hundred and forty six cases (50.6%) were females, while 239 cases (49.2%) were males. Majority of the cases were located in the mandibular premolar-molar region (29.0%). The predominant presenting symtom was swelling (94.0%). Majority of the cases (64.7%) showed multilocular radiolucency. The most common histologic pattern was plexiform (25%) followed by follicular pattern (23.5%). In conclusion, our data were comparable to those of previously reported cases of ameloblastoma with only minor differences.
7
Page : 357-365

Title : Oncocytoma : Report of 2 cases

Author(s) : Vichittra Vipismakul, Somchai Setthasirisombut, Prasan Tungjaturonrusmee, Sirinuch Jantarawattana

Keyword(s) : benign tumor of salivary gland,granular eosinophilic cytoplasm,intracytoplasic mitochondria,oncocyte,oncocytoma.

Manuscript Type : Case Report

Page : 357-365

Oncocytoma is a rare benign tumor of salivary gland. Two cases of oncocytoma are reported, one of which was presented as a slow growing mass of left submandibular area. The second case the lump located anterior to the right ear and extended to the angle of the mandible. Both cases were treated by surgical excision. Microscopically, the tumor mass consisted of large polyhedral cells with granular eosinophilic cytoplasm. The histochemical stain phosphotungstic acid hematoxylin was useful to confirm the diagnosis of oncocytoma, highlighting the intracytoplasmic mitochondria. The patients were free of recurrence after 9 and 1.5 years, respectively.
8
Page : 366-375

Title : Pain and anxiety control in anxious patients undergoing third molar surgery

Author(s) : Peera Jarearnsuk, Prisana Pripatnanont, Mayuree Wasinanukorn, Ureeporn Lekkat, Pornpan Danworanan, Poungjit Tintara

Keyword(s) : anxiety, conscious sedation, pain, third molar surgery

Manuscript Type : Original Article (บทวิทยาการ)

Page : 366-375

The purpose of this study was to compare two regimens used for anxiety and paincontrol in anxious patients undergoing third molar surgery. A randomized double blind clinical trial was performed in 2 groups with 15 patients in each group. Patients were randomly allocated to either the midazolam-opioid (fentanyl 1 mcg/kg intravenously) group or the midazolam-NSAIDs (naproxen sodium 550 mg. orally) group. The maximum dose of midazolam was limited to 0.1 mg/kg. Assessment compared sedation level, operation condition, recovery time, vital signs, oxygen saturation, amnesia, postoperative swelling, complication, and postoperative pain. The results showed that sedation level and operation in the midazolam-naproxen group were at optimal level and post-operative pain control was statistically significant better than the midazolam-fentanyl group (p<0.05). There were no statistical differences in amnesic effect, postoperative and swelling (p<0.05). The changes in vital signs and oxygen saturation did not affect the patients clinically. Minor intra-operative and postoperative complications were found in both groups. In conclusion, midazolam combined with NSAIDs appeared to be an alternative model, which is safe and effective for controlling pain and anxiety in anxious patients undergoing moderately difficult third molar surgery.
9
Page : 376-383

Title : Blood pressure and heart rate fluctuations in normotensive patients during extration

Author(s) : Kobsuke Sombatpium, Siriwan Sappalaek

Keyword(s) : -

Manuscript Type : Original Article (บทวิทยาการ)

Page : 376-383

The purpose of this study was to evaluate Blood pressure and heart rate fluctuations in normotensive patients during 5 inerval: 1) 5 minutes before procedure; 2) during injection of local anesthesia; 3) 5 minutes after injection; 4) during extraction and 5) 5 minutes after extraction. Blood pressure measurements were recorded at maximum baseline of 140/90 mmHg. Analysis using ANOVA for repeated measurement and SNK Comparisons were used in this study. there was no different in age between sex, therefore the results were reported as the total of 55 study subjects. The highest fluctuations of heart rate and blood pressure were noted during the period of extraction. They were also risen during administration of local anesthesia. Both blood pressure and heart rate were declined to near baseline at the time of 5 minutes after procedure. The significance of this findings is that there are maximal fluctuation during extraction, the highest stress response even in nomotensive patients which may present clinical risk during the dental stress associated with certain procedures.
10
Page : 384-390

Title : Fluoride content of foods and beverages

Author(s) : Em-on Benjavongkulchai, Marasri Ujjin

Keyword(s) : beverages,fluoride content,food,water

Manuscript Type : Original Article (บทวิทยาการ)

Page : 384-390

Information on fluoride content in food, water, beverages and consumed products is important to dentists in order to efficiently prescribe fluoride supplementation to patients. The purpose of this study is to report the current data on fluoride content in various locally consumed products including fruits (13 kinds), vegetables (9 kinds), seasoning sauces (6 brands), milk (3 brands), bottled water (9 brands), coconut milk (2 brands) and a variety of beverages (21 brands). Total or ionized fluoride concentration was determined using a fluoride electrode. It was found that most fruits tested had fluoride concentrations less than 0.02 ppm while vegetables had higher fluoride concentrations ranging from 0.022 to 1.066 ppm. Most of the tested products had low fluoride contents (<0.3 ppm) except tea drinks, one brand of orange juice and two brands of bottled water.
11
Page : 391-405

Title : The pilot study of association between the quantities of five periodontopathic pathogens and the Levels of periodontal pocket depth

Author(s) : Suttichai Krisanaprakornkit, Sakornrat Khongkhunthian, Teerawich Chotipanich, Rattana Umpriwan

Keyword(s) : periodontitis,periodontal pathogens,pocket depth,polymerase chain reaction

Manuscript Type : Original Article (บทวิทยาการ)

Page : 391-405

The objective of this study was to determine the association between the quantities of five periodontal pathogens and the levels of probing depth, indicative of the level of periodontal destruction. Subgingival plague samples were collected using paper points from both shallow (≤ 4 mm) and deep (≥ 6 mm) pockets of six aggressive periodontitis (AP) and six chronic periodontitis (CP) patients. Microbial DNA was isolated according to a standard protocal, and amplified by polymerase chain reaction (PCR) with specific primer pairs for individual microorganisms. Densitometry was conducted to analyze the intensities of PCR products, as a representative of the quantities of pathogens, from both shallow and deep pockets. The results showed that all microbial DNA tested, including Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.), Prevotella intermedia (P.i.), Bacteroides forsythus (B.f.), Treponema denticola (T.d.), could be identified by PCR technique. The median intensities of P.g.,B.f., and T.d. were significantly associated with the deep pocket in both AP (p=0.001, 0.001 and 0.001 respectively) and CP (p=0.001, 0.007 and 0.017 respectively). patients, while those of P.i. and A.a were significantly associated with the deep pocket only in AP patients (p=0.001 and 0.017 respectively). The median intensities of P.i. and A.a. were not significantly associated with the deep pocket in CP patients (p=0.114 and 0.887 respectively). Our data suggest that the quantities of P.g., B.f., and T.d. are associated with periodontal destruction in both AP and CP and may be used as a prognostic marker for the severity and progression of periodontal disease.
12
Page : 406-419

Title : simvastatin reduced the concanavalin-A-enhanced total MMP-9 expression in U2-OS osteoblast cell line

Author(s) : Pasutha Thunyakitpisal

Keyword(s) : concanavalin-A,MMP-9,osteoblast,simvastatin

Manuscript Type : Original Article (บทวิทยาการ)

Page : 406-419

MMP-9 (gelatinase-B) is a member of enzyme matrix metalloproteinage group (MMPs) that has been associated with the bone destructive diseases. The objective of this study was to investigate the effect of concanavalin-A and simvastatin on the expression of MMP-9 in U2-OS osteoblast cell line. Cells were treated with the increasing concentrations of concanavalin A with or without simvastatin for 24 and 48 hours. After that the conditioned media were collected. The MMP-9 expression was measured by using the gelatin zymography and western blot analysis. The results revealed that concanavalin-A, at 4, 6, 8 and 10 µg/ml, significantly increased the total expression of MMP-9 (p<0.01) at 24 hours. Conversely, simvastatin, at 10 mM, significantly reduce the cnocanavalin-A-enhanced total MMP-9 expression in U2-OS cells (p<0.05) at 48 hours. This data suggests that concanavalin-A, at 4, 6, 8 and 10 µg/ml stimulated the total expression of MMP-9, while simvastatin, at 10 mM, decreased the concanavalin-A-enhanced total MMP-9 expression in U2-OS cells.
14
Page : 424-427

Title : แนวคิดการร้องเรียนทันตแพทย์: กรณีศึกษาจากการร้องเรียนแพทย์

Author(s) : ปรียานุช บทกลอน, การุญ เลี่ยวศรีสุข

Keyword(s) : -

Manuscript Type : Miscellaneous (ปกิณกะ)

Page : 424-427

-
1
Page : 219-228

Title : Effect of fluoride releasing materials on demineralization of adjacent white spot lesion: in vitro study

Author(s) : Ruedee Sumalnop, Watcharaporn Tasajan, Chaiwat Maneenut

Keyword(s) : calcium,demineralization,fluoride releasing materials,pH-cycle,white spot lesion

Manuscript Type : Original Article (บทวิทยาการ)

Page : 219-228

The objective of this in vitro study was to investigate the effect of three fluoride releasing restorative materials under artificial caries process at the proximal of enamel by atomic absorption spectrophotometer to analyze the amount of calcium released form specimen as an indirect method. Sixty specimens from permanent molars were sized into 2x4 square millimeters immersed in demineralizing solution for 96 hours to create subsurface white spot lesions on enamel. The enamel specimens were distributed into four groups by using the amount of calcium in demineralizing solution analyzed by atomic absorption spectrophotometer. Then four restorative materials mounted into direct proximal contact with enamel specimens to imitate proximal filling. The control group was non-fluoride containing resin composite (Z 250®) and the three experimental groups were resin-modified glass ionomer cement (Fuji II LC® capsule), polyacid-modified resin composite (Compoglass® Flow) and fluoride containing resin composite (Heliomolar®). Then all the specimens were simulated oral environment cycle in acid for 2.5 hours and in remineralizing solution for 21.5 hours, daily for 30 days. After pH-cycle, the enamel specimens were separated from materials and immersed in demineralizing solution for 96 hours. The atomic absorption apectrophotometer was used to measure the amount of calcium. Mann-Whitney U Test and Kruskal-Wallis H Test were set to analyzed the difference of calcium releasing form enamel at p<0.05. The result showed that the average amount of calcium of specimen which was contact to Fuji II LC® capsule and Compoglass® Flow were significant less than Z 250® (p<0.05). While Heliomolar® group was significant greater than Fuji II LC® capsule and Compoglass® Flow (p<0.05). Therefore, the more releasing fluoride from material, the more effect on demineralization inhibition of adjacent white spot lesion. Nevertheless further laboratory and clinical study should be conducted. 
2
Page : 229-237

Title : Effect of fluoride mouthrinse on remineralization of enamel lesion: in vitro study

Author(s) : Praphasri Rirattanapong, Katkao Vongsavan, Rudee Surarit, Nopawan Juanarongrit

Keyword(s) : fluoride, mouthrinse, remineralization

Manuscript Type : Original Article (บทวิทยาการ)

Page : 229-237

The purpose of this study was to evaluate the effective of fluoride mouthrinse, oral med, xylitol, fluocaril and sodium fluoride on remineralization of enamel lesions. Preparation artificial caries lesions were formed in thin section of teeth for 75 specimens. The specimens were randomized into five groups, four experimental groups and one control group, 15 specimens for each group in pH cycling model. The specimens were immersed in demineralizing solution for 3 hours/day, 5 mins immersion in the test of fluoride mouthrinse, and 21 hours in remineralizing solution. The continuous cycles of demineralization and remineralization were carried out for 5 weeks. After experiment were finished, the depth of lesion was evaluated before treatment and after treatment by polarized light microscope. The depth of lesion in Oralmed kid® group had fluoride 500 ppm was significantly less than that of other fluoride mouthrinses. (p<0.05).
3
Page : 238-252

Title : Permanent first molar band sizes in a group of Thai orthodontic patients

Author(s) : Chintana Sirichompun, Chutima Srivanichakorn, Temsiri Porpan

Keyword(s) : permanent first molar,band size,frequencies,distribution,Thais

Manuscript Type : Original Article (บทวิทยาการ)

Page : 238-252

Almost all of the patients treated with fixed orthodontic appliances were assigned with permanent first molar bands. Manufacturers distributed the bands by using data obtained from Caucasian patients, which may be different from Thais. The purposes of this study were to determine the frequencies and modes of permanent first molar band sizes used in Thai orthodontic patients, as well as to compare the band size differences between gender, right- and left-sided teeth, maxillary and mandibular teeth, and Thais and Caucasians. A retrospective study was investigated in 300 orthodontic patient records (116 males and 184 females). All of the patients were treated in Graduate Clinic, Department of Orthodontic, Faculty of Dentistry, Chulalongkorn University during December 1991 to April 2003, and their four permanent first molars were delivered with the bands. Descriptive statistics were used to elucidate the frequencies and modes of the band sizes and to compare the data obtained between gender. The details between right-and left-sided teeth or between maxillary and mandibular teeth were compared by an analytic statistic using Sign Test. A comparison between Thais and Caucasians was performed by Kolmogorov-Smirnov Two-Sample Test. The frequencies were disclosed. The modes of #16, #26 and #36 band sizes were 36.5, while those of #46 were 36.0 and 37.0. Differences in the band sizes between gender were observed. A comparison between right- and left-sided teeth revealed no significant difference. However, the band sizes were significantly different (p<0.0001) between maxillary and mandibular teeth, and between Thais and Caucasians. Therefore, the decision on a purchase of such bands for Thais should be based on the results of study in Thais. Tables and graphs with distribution of permanent first molar band sizes were shown to aid orthdontists in band purchasing.
4
Page : 253-261

Title : Discontinuation of orthodontic treatment and related factors

Author(s) : Supanee Suntornlohanakul, Ratchada Tantisarasart

Keyword(s) : Discontinuation of orthodontic treatment,orthodontic patient

Manuscript Type : Original Article (บทวิทยาการ)

Page : 253-261

Discontinuation of orthodontic treatment is one of problems in the aspect of quality assurance audit and may also cause the waste of the resource. This study was intended to survey the prevalence of the orthodontic discontinuation rate and the relating factors. It was found that 27.5% of the patients discontinued their orthodontic treatment from the Dental Hospital, Faculty of Dentistry, Prince of Songkla University. The problems occurred in female more than in male and student were the most frequent among the other occupations. 27.6% of the patients visited orthodontic only first visit. The major problems for discontinuation was inconvenience. It is the orthodontist responsibility to be aware of this specific problem, and should carefully inform the treatment process to the patient before treatment and once the patient has started treatment, the orthodontist should try to motivate the patient to continue his/her treatment until treatment is finished.
5
Page : 262-270

Title : Trauma induced pseudogout of the temporomandibular joint: Report of a case and review of the literature

Author(s) : Pornchai Jansisyanont, Dr. Robert A. Ord

Keyword(s) : calcium pyrophosphate dihydrate,chondrocalcinosis,crystal deposition desease,pseudogout,temporomandibular joint

Manuscript Type : Original Article (บทวิทยาการ)

Page : 262-270

Pseudogout is the gout-like acute arthritis that is caused by the deposition of calcium pyrophosphate dihydrate (CPPD). The incidence of pseudogout is 5% of the population and about 50% of all cased occur in the knee joint, wrist and hand. wrist of the temporomandibular joint is rare. We reported a case with the history and clinical findings that led to the diagnosis of trauma induced pseudogout of the temporomandibular joint after other conditions had been ruled out. The surgical findings were useful to make the correct diagnosis and prevent the delay in management of the crystal induced arthropathies. The etiology, clinical manifestration, and management of this lesion were also reviewed.
6
Page : 271-275

Title : Adenoid squamous cell carcinoma: A case report

Author(s) : Kittipong Dhanuthai, Somporn Swasdison

Keyword(s) : Adenoid squamous cell carcinoma,cytokeratin,desmosome,pseudoglandular space

Manuscript Type : Case Report

Page : 271-275

Adenoid squamous cell carcinoma (ASCC) is an uncommon variant of squamous cell carcinoma commonly encountered in the sun-exposed areas of the head and neck region in middle-aged to elderly patients. This case presented ASCC in a 67 year-old male patient at unusual intraoral sites. Histologically, it showed features of squamous cell carcinoma coupled with pseudoglandular spaces containing detached acantholytic cells with no evidence of epithelial mucin. Ultrastructurally, these tumor cells showed intercellular desmosomes and intracellular tonofilaments. Immunohistochemically, the tumor cells expressed cytokeratin, but lacked endothelial cell marker. This case was treated by surgery with no sign of recurrence after a 2-year follow-up period. The clinical data of this case were compared with those previously reported cases. The differential diagnoses of this case were also discussed.
7
Page : 276-285

Title : Tooth wear Part I: Prevalence and etiology

Author(s) : Nattaya Asawaworarit, Jurai Nakaparksin, Nantana Traiyalaksana

Keyword(s) : dental abrasion,dental attrition,dental erosion,prevalence,tooth wear

Manuscript Type : Review Article (บทความปริทัศน์)

Page : 276-285

Tooth wear is an all-embracing term used to describe the combined process of erosion, attrition and abrasion or when the specific diagnosis cannot be determined. At the present time the definite prevalence of tooth wear is unknown but it is showing a tendency to increase particularly in teenagers and adolescents. Prevalence of tooth wear is increasing with increasing age especially on occlusal/ in and cervical tooth surfaces. Prevalence and severity of tooth wear on the occlusal/ incisal surfaces is the highest of all the surfaces especially in the elderly. The wear of lingual surface of maxillary anterior teeth is the most common in teenager group but it does not relate to the increasing age. Tooth wear in adult is the combined process of erosion attrition and abrasion, but the wear of teeth in teenagers is always erosion related to the consumption of acidic soft drinks. Etiology of tooth wear was also discussed.
1
Page : 141-153

Title : Forces generated by various types of orthodontic leveling archwires

Author(s) : Chintana Sirichompun, Vachara Phetcharakupt

Keyword(s) : archwires,forces,leveling,rank

Manuscript Type : Original Article (บทวิทยาการ)

Page : 141-153

 
The purposes of this study were to compare and rank the forces generated by seven types of orthodontic leveling archwires, which possessed various cross-sectional areas (CA), at each level of tooth irregularity (TI). The archwires used in this study classified into 23 groups and their number in each group was 20. Using Lloyd universal testing machine LR 10K, an in vitro study by a bending test of each archwires was performed in the lower arch epoxy resin dentoform. The forces were analytically compared by an Independent-Sample T test and a One-Way ANOVA at p=0.05. At different level of TI, the forces generated by the same type of archwire with the same CA were significantly different (p>0.001). In addition, a heavier force was transferred to the tooth with a more irregularity than that with a less irregularity. At different CA but the same level of TI, the forces generated by the archwire with a larger CA, but not by Tripleflex .0150 and .0175 inch at TI=0.5, 1.0 and 1.5 mm (p=0.954, 0.122 and 0.224), by D-Rect 0.17x.025 and .018x.025 inch² at TI=0.5, 1.0, 1.5 and 2.0 mm (p=1.00, 1.00, 1.00 and 1.00), by NiTi .018 inch and .017x0.175 inch² and TI=0.5 and 1.5 mm (p=0.998 and 0.323), or by NiTi .0175x.025 and .018x.025 inch² at TI=1.0 and 1.5 mm (p=0.978 and 0.063), respectively. At the same CA and level of TI, the forces generated by different types of archwires were significantly different (p<0.001), expect NiTi and TMA .018 inch at TI=0.5 mm (p=0.175). Tables and a graph with a rank of forces generated by 23 groups of archwires were shown to aid orthodontists during wire selection and in wire progression.
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Page : 154-160

Title : Expression of tumor necrosis factor-alpha (TNF-α) in trigeminal neuralgia patients: a preliminary report.

Author(s) : Mr. Siriporn Chattipakorn, Surawut Pongsiriwet, Suttichai Krisanaprakornkit, Nipon Chattipakorn

Keyword(s) : ELISA, pain, TNF-α trigeminal neuralgia

Manuscript Type : Original Article (บทวิทยาการ)

Page : 154-160

Trigeminal neuralgia is one of the most common neuropathic pain found in Dentistry. The cause of this neuralgia is still unknown. It has been suggested that neuroimmunological mechanisms may be involved in the development and maintenance of neuropathic pain. Increase in the level of proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α), have been intricately linked with the pathogenesis of several animal models of neuropathic pain. In this study, we investigate whether the proinflammatory cytokine, particularly TNF-α, acts as a pain mediator in the trigeminal neuralgia in humans. Blood samples were drawn from patients during painful and pain-free episode of trigeminal neuralgia patients. The expression of TNF-α in serum was analyzed by an enzyme-linked-immunosorbent assay (ELISA). We found that the TNF-α levels in serum from all patients (N=6) during painful episodes tended to be elevated compared to pain-free episodes in the same patients. Interestingly, total white blood count (WBC), especially number of neutrophils and lymphocytes, also increased during painful episodes. Our finding suggests that it is possible that the alteration in TNF-α expression may be associated with painful episodes of the trigeminal neuralgia, similar to other types of neuropathic pain. Therefore, TNF-α may be a new therepeutic target for trigeminal neuralgia.Trigeminal neuralgia is one of the most common neuropathic pain found in Dentistry. The cause of this neuralgia is still unknown. It has been suggested that neuroimmunological mechanisms may be involved in the development and maintenance of neuropathic pain. Increase in the level of proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α), have been intricately linked with the pathogenesis of several animal models of neuropathic pain. In this study, we investigate whether the proinflammatory cytokine, particularly TNF-α, acts as a pain mediator in the trigeminal neuralgia in humans. Blood samples were drawn from patients during painful and pain-free episode of trigeminal neuralgia patients. The expression of TNF-α in serum was analyzed by an enzyme-linked-immunosorbent assay (ELISA). We found that the TNF-α levels in serum from all patients (N=6) during painful episodes tended to be elevated compared to pain-free episodes in the same patients. Interestingly, total white blood count (WBC), especially number of neutrophils and lymphocytes, also increased during painful episodes. Our finding suggests that it is possible that the alteration in TNF-α expression may be associated with painful episodes of the trigeminal neuralgia, similar to other types of neuropathic pain. Therefore, TNF-α may be a new therepeutic target for trigeminal neuralgia.
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Page : 161-167

Title : The study of fluoride toothpaste ingestion in preschool children

Author(s) : Wikul Visalseth, Nontinee Tangchareondee, Surang Chettaprin, Suwipa Anantanasawat

Keyword(s) : fluoride intake,preschool children,toothpaste ingestion

Manuscript Type : Original Article (บทวิทยาการ)

Page : 161-167

This study aimed to determine the amount of toothpaste ingested during tooth brushing in children aged 3-6. The 120 children brushed their teeth with toothpaste containing fluoride 1,000 ppm. The amount of toothpaste used was 0.22 grams. Fluoride content of collected rinsing fluid was determined by fluoride-ion-specific electrode and was used to estimate the amount of toothpaste ingested. Result showed that 55% of children swallowed a quarter to half of the toothpaste used and 5.8% swallowed more than half. The average amount of toothpaste ingested was 29.71% w/w. The older a child was, the less amount of toothpaste ingested (p<0.05). The average amount of fluoride intake from toothbrushing two times a day was 0.0065 mgF/Kg bw/day.This study aimed to determine the amount of toothpaste ingested during tooth brushing in children aged 3-6. The 120 children brushed their teeth with toothpaste containing fluoride 1,000 ppm. The amount of toothpaste used was 0.22 grams. Fluoride content of collected rinsing fluid was determined by fluoride-ion-specific electrode and was used to estimate the amount of toothpaste ingested. Result showed that 55% of children swallowed a quarter to half of the toothpaste used and 5.8% swallowed more than half. The average amount of toothpaste ingested was 29.71% w/w. The older a child was, the less amount of toothpaste ingested (p<0.05). The average amount of fluoride intake from toothbrushing two times a day was 0.0065 mgF/Kg bw/day.
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Page : 168-184

Title : Collagen and gingival fibroblasts activate gelatinases in oral aquamous carcinoma cell lines

Author(s) : Neerach Sanchavanakit, Suchart Kanwasukul, Sangkhom Phimlee, Artiphan Pimkhaokham, Prasit Pavasant

Keyword(s) : HGF, HSC, MMP-2 activation, type I collagen gel

Manuscript Type : Original Article (บทวิทยาการ)

Page : 168-184

The aim of this study was to investigate the influence of type I collagen gel and human gingival fibroblasts (HGF) on human oral squamous carcinoma cell lines (HSCs) for the activation of gelatinases. The studies were conducted in two parts. For the first part, HSC-3 and HSC-7 were cultured on type I collagen gel for 48 hours. The levels of gelatinases were analysed by zymography, while the level of MT1-MMP mRNA was determined by RT-PCR. In addition, cell morphology on collagen gel was examined under the microscope. HSC-3 and HSC-7 demonstrated different morphological change and arrangement when cultured on collagen gel. However, induction of the MMP-2 activation was found in both cell types, which corresponded to the up-regulation of MT1-MMP mRNA. In the second part, HSC and HGF were co-cultured in the condition of cell-cell contact or non-contact for 24 hours and levels of gelatinases were examined by zymography. MMP-2 activation was noted when HSC and HGF were in contact. In conclusion, type I collagen gel and human gingival fibroblasts could enhance the virulence of HSC-3 and HSC-7 by inducing MMP-2 activation, which is one of the important step in metastasis of cancer cells.
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Page : 185-195

Title : Effects of DFDB and FDB on differentiation of PDL cells in vitro

Author(s) : Tussanee Yongchaitrakul, Siriluk Tiranathanagul, Prasit Pavasant

Keyword(s) : DFDB, FDB, PDL cells

Manuscript Type : Original Article (บทวิทยาการ)

Page : 185-195

A purpose of the study was to compare the response of human PDL cells to demineralized freeze-dried bone (DFDB) and freeze dried bone (FDB). We aimed at the responses of healing, cell differentiation and mineralization. Human PDL cells were cultured in the presence of DFDB or FDB in different timing compared with the control. Cell proliferation was assayed by methylene blue and fibronectin synthesis was analyzed by Western analysis. The expression of osteopontin and bone sialoprotein were analyzed by RT-PCR and calcification was detected by calcium staining. The results showed that DFDB could stimulate cell proliferation and fibronectin synthesis at short term. Cells responded to DFDB by increasing osteopontin and bone sialoprotein expression at day 4, which was likely a short term response. To compare with DFDB, FDB treated cultures also showed the increase of both proteins expression but required a longer period, day 8 and 18. In addition, calcification was detected in FDB cultures at day 18. In conclusion, DFDB and FDB could result in increasing of osteopontin and bone sialoprotein expression in human PDL cells. An increase of both proteins was not concurrent with calcification in vitro. However, the increase of the proteins demonstrated cell response in the aspect of differentiation. The results direct demonstrated that both DFDB and FDB played roles in helping periodontal regeneration. It could be an effect in a short term seen in DFDB culture or a long-term effect found with FDB.
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Page : 196-209

Title : Repairing of fractured porcelain metal-ceramic restorations. 1. Surface treatment of porcelain and alloy

Author(s) : Boonlert Kukiattrakoon

Keyword(s) : alloy,porcelain,repair,surface treatment

Manuscript Type : Review Article (บทความปริทัศน์)

Page : 196-209

The objective of this article was to explain the surface treatment of porcelain and alloy for repairing of fractured porcelain metal-ceramic restorations. Because of making a new restoration is difficult methods, loss time and economic crisis. So repairing in an alternative choice for the patient. The important factor is the surface treatment of porcelain that can be used both mechanical retention such as grinding with a course diamond bur, sandblast, etching with bydrofluoride acid or acidulated phosphate fluoride gel and chemical bonding such as silane. Similarity for alloy, mechanical retention such as grinding with a carborundum disc or a course diamond bur, sandblast, chemical etching or electrolytic etching and chemical bonding such as tin plate, silica coated aluminium oxide, primer and silane. In addition, this article also included the surface treatment of choice in clinical practice for porcelain and alloy.
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Page : 210-218

Title : Is it the time for dental digital radiography

Author(s) : Sittidej Nincharoen

Keyword(s) : advantages,diagnostic root,digital radiography,disadvantages.

Manuscript Type : Review Article (บทความปริทัศน์)

Page : 210-218

The use of radiographs as a diagnostic tool has become an indispensable routine in medicine and dentistry. For a long time, radiographic film has been the most important medium to acquire and archive the diagnostic image. In 1987, the first intraoral RadioVisioGraphy (RVG) system was introduced which included a charge coupled device (CCD) sensor and processing unit with the digital image display on a monitor. There are many different ways to acquire a digital image. Mainly are two different ways: indirect and direct. In indirect digital imaging, an actual film-based radiograph is still required. Then an imaging device such as scanner or video camera is used to scan or photograph the film-based radiograph. In direct digital imaging, no film is used. The digital image is acquired directly by means of a sensor. There are currently three types of sensors commonly used: Charge Coupled Device (CCD), Complimentary Metal Oxide Semiconductor (CMOS) and Photo Stimulable Phosphors (PSP). There are several advantages of digital radiography mostly based on the reduction of radiation exposure level and the elimination of the photographic process. In addition to viewing the images immediately after exposure, they will allow the use of various image-processing tools that can enhance and improve images for better diagnosis and patient education. However, no system is perfect, therefore it currently has some disadvantages. The disadvantages are diminishing as technology advantages. The most important prerequisite for meaningful use of digital radiography in the dental practice is supporting instead of disturbing daily clinical work. The underlying software should be easily comprehensible and intuitively applicable. Despite the disadvantages, digital radiography represents the new age of imaging in dentistry. Since the past few years, although film-based radiography has not been abandoned completely with rapidly developing technology a number of digital radiography is now well accepted modality and increased rapidly. The purpose of this paper will describe the advantages and disadvantages of digital radiography and compare to those of conventional radiography. How the introduction of digital radiography into dental practice affects clinical work is also discussed. Therefore, the dentists can make their own decisions whether to start or not.
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Page : 71-78

Title : The Retention of Local Made Sealant (a Clinical Study: 36-Month Follow-up)

Author(s) : Siriruk Nakornchai, Wanchai Chaweekulrat, Piyawat Keingthong

Keyword(s) : local made sealant, retention, clinical study

Manuscript Type : Original Article (บทวิทยาการ)

Page : 71-78

The objectives of this study were to compare clinical retention of Local Made Sealant (MTEC clear) and imported sealant (Delton clear) and to examine dental caries after sealant loss. The sealant was applied randomly on 150 first permanent molars using a half-mouth design. The retention was investigated as total retention, partial loss, and total loss. The retention of two sealants was compared using Chi-square test. When considering the sealant retention on occlusal surface, there, there was significant difference at 12, 24, 30 and 36 months, respectively (p=.027, .003, .000, .0030). The retention of sealant on occlusal surface and buccal pit and groove of lower molars or lingual groove of upper molars were significantly different at 12, 18, 24, 30 and 36 months, respectively (p=.020, .006, .027, .000, .003). The retention rate of Delton was higher than local made sealant. The carious lesion was detected on occlusal surface of teeth previously sealed with MTEC and Delton at 24 and 30 months, respectively. Buccal or lingual pit caries of the two sealants were found at 24 months.The objectives of this study were to compare clinical retention of Local Made Sealant (MTEC clear) and imported sealant (Delton clear) and to examine dental caries after sealant loss. The sealant was applied randomly on 150 first permanent molars using a half-mouth design. The retention was investigated as total retention, partial loss, and total loss. The retention of two sealants was compared using Chi-square test. When considering the sealant retention on occlusal surface, there, there was significant difference at 12, 24, 30 and 36 months, respectively (p=.027, .003, .000, .0030). The retention of sealant on occlusal surface and buccal pit and groove of lower molars or lingual groove of upper molars were significantly different at 12, 18, 24, 30 and 36 months, respectively (p=.020, .006, .027, .000, .003). The retention rate of Delton was higher than local made sealant. The carious lesion was detected on occlusal surface of teeth previously sealed with MTEC and Delton at 24 and 30 months, respectively. Buccal or lingual pit caries of the two sealants were found at 24 months. 
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Page : 79-90

Title : The Accuracy of Root ZX Apex Locator in Primary Teeth

Author(s) : Onauma Angwaravong, Sommai Chobisara, Piyanee Panitvisai

Keyword(s) : Electronic apex locator,primary teeth,Root canal determination,Root ZX

Manuscript Type : Original Article (บทวิทยาการ)

Page : 79-90

The purpose of this in vitro study was to test the accuracy of Root ZX by comparing the tooth length measured by Root ZX apex locator reading at "Apex" with the real tooth length. One hundred and twenty-four extracted primary molars were divided into two equal groups according to the degree of root resorption: group 1; root resorption<1/6 of root length, group 2; root resorption 1/6-1/3 of root length. The tooth length measured by both methods were compared and analyzed statistically by paired t-test. The results showed no statistically significant difference between the two methods (p>0.05). Root ZX was able to locate the foramen within a range of 0.5 mm in 60 out 62 teeth which was the accuracy rate of 96.77% in both groups. Mean differences between the two methods were 3.39x10ˉ² ±0.19 mm for group 1 and 0.72x10ˉ² ±0.23 for group 2.The purpose of this in vitro study was to test the accuracy of Root ZX by comparing the tooth length measured by Root ZX apex locator reading at "Apex" with the real tooth length. One hundred and twenty-four extracted primary molars were divided into two equal groups according to the degree of root resorption: group 1; root resorption<1/6 of root length, group 2; root resorption 1/6-1/3 of root length. The tooth length measured by both methods were compared and analyzed statistically by paired t-test. The results showed no statistically significant difference between the two methods (p>0.05). Root ZX was able to locate the foramen within a range of 0.5 mm in 60 out 62 teeth which was the accuracy rate of 96.77% in both groups. Mean differences between the two methods were 3.39x10ˉ² ±0.19 mm for group 1 and 0.72x10ˉ² ±0.23 for group 2. 
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Page : 91-97

Title : The Use of An Anterior Jig in Restoration of Vertical Dimension of Occlusion

Author(s) : Rapeephan Nagasiri, Premwara Triwatana

Keyword(s) : anterior jig,centric relation,maxillomandibular relation,vertical dimension of occlusion

Manuscript Type : Original Article (บทวิทยาการ)

Page : 91-97

Full-mouth prosthodontic rehabilitation usually involved establishing of an occlusion at a new vertical dimension. The use of an anterior jig helps determine vertical dimension of occlusion in the physiologic position. This device assists in managing the mandible to the hinge rotation position without any influences from occluding teeth and also functions as an anterior stop, thus, achieving maxillomandibular relationship in centric relation at the determined vertical dimension of occlusion. This article presents the anterior jig fabricating procedures and case applications. 
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Page : 98-102

Title : Oral Melanoma: An Immunohistochemical and Electron Microscopic Studies

Author(s) : Suparp Kietthubthew, Supis Chungpanich, Winyou Mitarnum, Sakol Suwaluk, Jarinda Thaisangsa-nga

Keyword(s) : electron microscopy, immunohistochemistry, malignant pigmented lesion, oral melanoma, palatal mass

Manuscript Type : Original Article (บทวิทยาการ)

Page : 98-102

Oral melanoma is an uncommon disease. It accounts for 1-8% of all melanomas. We reported a case of a 68-year-old Thai male presented with a pedunculated pigmented nodular mass on his palate. He noticed the lesion as a small pale-colored exophytic mass three years ago. It grew gradually. The mass enlarged rapidly in the past few months without any remarkable symptoms. The clinical impression for this lesion was a malignant melanoma or a pyogenic granuloma with hemorrhage. Histopathological examination revealed an ill-defined lesion in the submucosa. It was compose mainly of pigmented cells with pleomorphic shape, and size. An immunohistochemical study showed positivity of s-100 and vimentin and negativity of cytokeratin, EMA, and CD68. Ultrastructurally, the cells demonstrated pleomorphic solitary melanosomes in the cytoplasm. The lesion was diagnosed as a malignant melanoma. He was treated with a wide excision. The patient lost to follow-up two months after surgery.Oral melanoma is an uncommon disease. It accounts for 1-8% of all melanomas. We reported a case of a 68-year-old Thai male presented with a pedunculated pigmented nodular mass on his palate. He noticed the lesion as a small pale-colored exophytic mass three years ago. It grew gradually. The mass enlarged rapidly in the past few months without any remarkable symptoms. The clinical impression for this lesion was a malignant melanoma or a pyogenic granuloma with hemorrhage. Histopathological examination revealed an ill-defined lesion in the submucosa. It was compose mainly of pigmented cells with pleomorphic shape, and size. An immunohistochemical study showed positivity of s-100 and vimentin and negativity of cytokeratin, EMA, and CD68. Ultrastructurally, the cells demonstrated pleomorphic solitary melanosomes in the cytoplasm. The lesion was diagnosed as a malignant melanoma. He was treated with a wide excision. The patient lost to follow-up two months after surgery.  
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Page : 103-117

Title : Development of Risk Criteria Related to Food Cariogenictiy

Author(s) : Sunee Wongkongkathep, Sutha Jienmaneechotchai, Supranee Dalodom, Voravithaya Chaimuang

Keyword(s) : children, cariogenicity, plaque pH, snacks

Manuscript Type : Original Article (บทวิทยาการ)

Page : 103-117

The aim of this study is to investigate the changes of dental plaque pH after exposing to snacks in order to develop risk criteria related to food cariogenicity. Snacks were selected from top 10 preferential snacks of primary school children, survey from 12 local stores. Snacks which claimed to be no cariogenicity were also included. Snacks were seperated into 13 groups containing 49 items. Four research teams are well trained and calibrated prior to the study. Twelve volunteers of 12 years of age who had DMFT < 4 were recruited in each team. Plaque pH was determined using a micro-glass combination electrode (Micro pH electrode #P13-4-5-BNC). Analysis of minimum pH and area under curve (pH<5.7), using one-way analysis of variance statistics (ANOVA) and Independent t-test. From this study, snacks could be classified in 4 groups according to their cariogenic potential as low, medium, high and very high levels. "Sun flower Kernels, Dried Squid snack, Fish Fiber snack 1-2, Papaya" were low risk group which are safe for tooth. "Kautommud, Peanut paste, Khanomtarn, Cookies coated milk, Thongmuan, Peanuts coated starch, Cookies sandwich, Cookies coated chocolate, Sweeten egg drop" were in the very high risk group.The aim of this study is to investigate the changes of dental plaque pH after exposing to snacks in order to develop risk criteria related to food cariogenicity. Snacks were selected from top 10 preferential snacks of primary school children, survey from 12 local stores. Snacks which claimed to be no cariogenicity were also included. Snacks were seperated into 13 groups containing 49 items. Four research teams are well trained and calibrated prior to the study. Twelve volunteers of 12 years of age who had DMFT < 4 were recruited in each team. Plaque pH was determined using a micro-glass combination electrode (Micro pH electrode #P13-4-5-BNC). Analysis of minimum pH and area under curve (pH<5.7), using one-way analysis of variance statistics (ANOVA) and Independent t-test. From this study, snacks could be classified in 4 groups according to their cariogenic potential as low, medium, high and very high levels. "Sun flower Kernels, Dried Squid snack, Fish Fiber snack 1-2, Papaya" were low risk group which are safe for tooth. "Kautommud, Peanut paste, Khanomtarn, Cookies coated milk, Thongmuan, Peanuts coated starch, Cookies sandwich, Cookies coated chocolate, Sweeten egg drop" were in the very high risk group.
6
Page : 118-130

Title : Facial Defects and Prosthetic Reconstruction current Concept

Author(s) : Piyawat Phankosol, Panarat Kodkeaw, Kussalee Wilairat, Niyom Thamronganansakul

Keyword(s) : craniofacial implant, facial defects, facial prosthesis, maxillofacial rehabilitation, prosthetic reconstruction

Manuscript Type : Review Article (บทความปริทัศน์)

Page : 118-130

 
Loss of a part of the face or having a congenital missing ear, nose, or eye is likely to have great effect on physical, psychological, and social impact of patient life. Prosthetic reconstruction is an acceptable alternative treatment and may be preferable to complex plastic surgical reconstruction. Most modern facial prosthesis is secured with adhesives. However, there are several disadvantages for the continual use of adhesives which can reduce their effectiveness and longevity. The use of osseointegrated implants to retain a facial prosthesis can alleviate these problems and improve the effectiveness for rehabilitating patients with extensive soft and hard tissue craniofacial defects.Loss of a part of the face or having a congenital missing ear, nose, or eye is likely to have great effect on physical, psychological, and social impact of patient life. Prosthetic reconstruction is an acceptable alternative treatment and may be preferable to complex plastic surgical reconstruction. Most modern facial prosthesis is secured with adhesives. However, there are several disadvantages for the continual use of adhesives which can reduce their effectiveness and longevity. The use of osseointegrated implants to retain a facial prosthesis can alleviate these problems and improve the effectiveness for rehabilitating patients with extensive soft and hard tissue craniofacial defects.
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Page : 131-140

Title : Techniques in Molecular Biology for Dental Research

Author(s) : Suttichai Krisanaprakornkit

Keyword(s) : Immunocytochemistry,Immunohistochemistry,in situ hybridization,Molecular Biology,Northern blot hybridization,Western blot hybridization

Manuscript Type : Review Article (บทความปริทัศน์)

Page : 131-140

The central dogma of Molecular Biology is transcription and translation. transcription is a process of RNA synthesis from DNA, whereas translation is a process of protein synthesis. Nowadays, knowledge and various techniques in Molecular Biology have increasingly become a useful tool for scientific experiments to understand molecular mechanisms of cell and organ development and disease pathogenesis. Conducting scientific research in Dentistry, which is part of Health Sciences, unavoidably requires basic are categorized according to their purposes, i.e. study of mRNA versus protein expression or in vitro versus in vivo. It is demanded for dental faculties and dentists to understand the principles of these techniques in which they can apply for their own research.The central dogma of Molecular Biology is transcription and translation. transcription is a process of RNA synthesis from DNA, whereas translation is a process of protein synthesis. Nowadays, knowledge and various techniques in Molecular Biology have increasingly become a useful tool for scientific experiments to understand molecular mechanisms of cell and organ development and disease pathogenesis. Conducting scientific research in Dentistry, which is part of Health Sciences, unavoidably requires basic are categorized according to their purposes, i.e. study of mRNA versus protein expression or in vitro versus in vivo. It is demanded for dental faculties and dentists to understand the principles of these techniques in which they can apply for their own research.
1
Page : 1-7

Title : The efficacy of an herbal-based toothpaste on the control of plaque and gingivitis

Author(s) : Thirapat Premsirinirund, Aree Jainkittivong

Keyword(s) : gingival indices, herbal-based toothpaste

Manuscript Type : Original Article (บทวิทยาการ)

Page : 1-7

The efficacy of an herbal-based toothpaste on the control of plaque and gingivitis was investigated in a double-blind study on 40 subjects aged 22-54 years. Over a period of 8 weeks, 20 subjects used either control or preparations containing herbal ingredients. Parameters measured were the plaque, gingival and bleeding indices. These examinations were determined at baseline, 2, 4, 6 and 8 weeks. The test and control toothpastes significantly reduced all parameters measured in comparison to the baseline values (p<0.0001) at all trial periods. In the present study, the herbal toothpaste was no more effective than the control toothpaste in reducing plaque and gingival inflammation. The test toothpaste exhibited slightly lower number of plaque, gingival and bleeding indices than the control toothpaste at all trial periods, but the differences were not statistically significant. Thus, the present results did not demonstrate a significant clinical advantage of the herbal-based toothpaste over the control toothpaste.The efficacy of an herbal-based toothpaste on the control of plaque and gingivitis was investigated in a double-blind study on 40 subjects aged 22-54 years. Over a period of 8 weeks, 20 subjects used either control or preparations containing herbal ingredients. Parameters measured were the plaque, gingival and bleeding indices. These examinations were determined at baseline, 2, 4, 6 and 8 weeks. The test and control toothpastes significantly reduced all parameters measured in comparison to the baseline values (p<0.0001) at all trial periods. In the present study, the herbal toothpaste was no more effective than the control toothpaste in reducing plaque and gingival inflammation. The test toothpaste exhibited slightly lower number of plaque, gingival and bleeding indices than the control toothpaste at all trial periods, but the differences were not statistically significant. Thus, the present results did not demonstrate a significant clinical advantage of the herbal-based toothpaste over the control toothpaste.
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Page : 8-18

Title : Comparison of Chlorhexidine mouthwash 0.12% and special mouthwash (chloroxylenol 0.004%): Efficiency in reducing saliva bacterial count

Author(s) : Kanokporn Pangsomboon, Duangrporn Kerdpon, Rawee Teanpaisan, Alan Geater

Keyword(s) : chlorhexidine,chloroxylenol,mouth rinse,mouth wash,saliva bacteria count,special moutwash

Manuscript Type : Original Article (บทวิทยาการ)

Page : 8-18

The current study aims to investigate the efficiency in reducing the saliva bacterial counts after rinsing a special mouthwash (chloroxylenol 0.0004%) when compared with Chlorhexidine mouthwash 0.12%. 104 dental students were divided into 4 groups for rinsing with 4 kinds of mouthwash; Chlorhexidine mouthwash 0.12%, chlorhexidine placebo, special mouthwash, and special mouthwash placebo. A 2 ml unstimulated saliva sample was collected; before and at 1, 30, 60 and 180 minutes after rinsing with each mouthwash. The saliva samples were then cultured in aerobic and anaerobic conditions and processed for differentiation and total bacterial counts. Chlorhexidine 0.12% mouthwash was found to be more efficient than special mouthwash in reducing saliva bacterial counts at every sample time point. The efficiency in reducing saliva bacterial count of special going dental procedures can reduce anaerobic bacteria, and it can last for up to 3 hours. This experiment shows that special mouthwash cannot replace Chlorhexidine mouthwash 0.12% in rinsing before undergoing dental procedures.The current study aims to investigate the efficiency in reducing the saliva bacterial counts after rinsing a special mouthwash (chloroxylenol 0.0004%) when compared with Chlorhexidine mouthwash 0.12%. 104 dental students were divided into 4 groups for rinsing with 4 kinds of mouthwash; Chlorhexidine mouthwash 0.12%, chlorhexidine placebo, special mouthwash, and special mouthwash placebo. A 2 ml unstimulated saliva sample was collected; before and at 1, 30, 60 and 180 minutes after rinsing with each mouthwash. The saliva samples were then cultured in aerobic and anaerobic conditions and processed for differentiation and total bacterial counts. Chlorhexidine 0.12% mouthwash was found to be more efficient than special mouthwash in reducing saliva bacterial counts at every sample time point. The efficiency in reducing saliva bacterial count of special going dental procedures can reduce anaerobic bacteria, and it can last for up to 3 hours. This experiment shows that special mouthwash cannot replace Chlorhexidine mouthwash 0.12% in rinsing before undergoing dental procedures. 
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Page : 26-36

Title : Estrogen enhances the effect of IL-1β and TNF-α on MMP-1 secretion and NOS-3 expression in human PDL cells.

Author(s) : Tussanee Yongchaitrakul, Prasit Pavasant

Keyword(s) : estrogen, IL-1, MMP-1, NOS, periodontal ligament cells, TNF

Manuscript Type : Original Article (บทวิทยาการ)

Page : 26-36

 
The purpose of this study is to investigate the responses of human periodontal ligament (HPDL) cells after activating with IL-1β and TNF-α alone of activating with the combination of estrogen. After 48 hours of activation, the levels of MMP-1 in the medium and NOS-3 expression were examined using western analysis and RT-PCR, respectively. The results indicated that of NOS-3. Interestingly, when cells were activated with estrogen and IL-1β simultaneously, the level of MMP-1 and NOS-3 increased up to 2.3 and 5 folds, respectively, compared with 1.3 and 4.2 folds when cells received IL-1β alone. Similarly, activation with estrogen and TNF-α increased the level of MMP-1 and NOS-3 from 2.2 and 1.8 folds up to 3.2 and 2.5 folds, respectively, in comparing with TNF-α treated alone. The results showed that increasing level of estrogen could enhance the effect of pro-inflammatory factors, which consequently enhanced periodontal tissue destruction.The purpose of this study is to investigate the responses of human periodontal ligament (HPDL) cells after activating with IL-1β and TNF-α alone of activating with the combination of estrogen. After 48 hours of activation, the levels of MMP-1 in the medium and NOS-3 expression were examined using western analysis and RT-PCR, respectively. The results indicated that of NOS-3. Interestingly, when cells were activated with estrogen and IL-1β simultaneously, the level of MMP-1 and NOS-3 increased up to 2.3 and 5 folds, respectively, compared with 1.3 and 4.2 folds when cells received IL-1β alone. Similarly, activation with estrogen and TNF-α increased the level of MMP-1 and NOS-3 from 2.2 and 1.8 folds up to 3.2 and 2.5 folds, respectively, in comparing with TNF-α treated alone. The results showed that increasing level of estrogen could enhance the effect of pro-inflammatory factors, which consequently enhanced periodontal tissue destruction. 
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Page : 37-50

Title : A Survey of musculoskeletal pain among Thai dentists and recommendations for prevention

Author(s) : Suwannee Tuongratanaphan, Songvuth Tuongratanaphan, Chintana Itthidecharon

Keyword(s) : dentists,musculoskeletal pain,occupational health,working posture

Manuscript Type : Original Article (บทวิทยาการ)

Page : 37-50

The aim of this was to obtain data on the prevalence of musculoskeletal pain among Thai dentists. Questionnaires were distributed to 1,197 Thai dentists. The overall response rate was 31.3 percent (375 subjects). 37.3 percent were male and 61.1 percent were female. 46.1 percent of the respondents have provided dental services more than 30 hours per week. For working posture, 60.3 percent did not usually used chair back rest and 45.6 percent sat with curve back. The study revealed that three most common location of musculoskeletal pain were lower back 82.1 percent, neck 60.5 percent and shoulder (backside) 48.8 percent. This study was a descriptive study and did not reveal relationships of any factor of musculoskeletal pain with the context of dental of dental work. However, this article also provided recommendations for prevention of musculoskeletal pain for dentists which would be beneficial to the profession.
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Page : 51-57

Title : Clinical evaluation of sealant bonding to partial eruption of the first permanent molars in 3 months

Author(s) : Pornpan Leewattana, Kutkao Vongsavan, Praphasri Porntaweewat, Penpak Apinantanawat

Keyword(s) : bonding agent, partial eruption, permanent molars, sealant

Manuscript Type : Original Article (บทวิทยาการ)

Page : 51-57

Pit and fissure sealants have been effective in reducing occlusal caries but their effectiveness on partially erupted teeth may be precluded by difficulties with moisture control and tissue management during application. In preliminary studies, a bonding agent has been shown to bond adequately to etched enamel after salivary contamination. The objective of this study was to evaluate clinical retention of sealant bonding eruption of the first permanent molars in 3 months. Thirty-six teeth in twenty-one children were divided randomly into two groups: sealant was placed with or without the bonding agent. 3M™ Single Bond. After 3 months, the retention rate for the sealant with Single Bond adhesive were 88.89% full retention and 11.11% partial loss versus 77.78% full retention 22.22% partial loss for sealant without bonding agent and there was no total loss in both groups. The retention of two groups was compared using Fisher Exact test. The results show that there was no significant difference between the two groups in 3 months (p=0.24).Pit and fissure sealants have been effective in reducing occlusal caries but their effectiveness on partially erupted teeth may be precluded by difficulties with moisture control and tissue management during application. In preliminary studies, a bonding agent has been shown to bond adequately to etched enamel after salivary contamination. The objective of this study was to evaluate clinical retention of sealant bonding eruption of the first permanent molars in 3 months. Thirty-six teeth in twenty-one children were divided randomly into two groups: sealant was placed with or without the bonding agent. 3M™ Single Bond. After 3 months, the retention rate for the sealant with Single Bond adhesive were 88.89% full retention and 11.11% partial loss versus 77.78% full retention 22.22% partial loss for sealant without bonding agent and there was no total loss in both groups. The retention of two groups was compared using Fisher Exact test. The results show that there was no significant difference between the two groups in 3 months (p=0.24).
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Page : 58-69

Title : Evaluation of new tooth brushing education program for primary school children

Author(s) : Narong Suksu-art

Keyword(s) : oral hygiene,plague,tooth brushing

Manuscript Type : Original Article (บทวิทยาการ)

Page : 58-69

This study aimed to evaluate the oral hygiene status of primary school after using a new tooth brushing education program. The 176 subjects were primary school, aged 11-12. Purposive sampling was used for school selection and simple random sampling was used for dividing children into 3 groups; experimental group (57) and 2 control groups, one belonged to the same school (58) as the experimental group and the other belonged to a different school (61). Data collection before implementation was done by using a questionnaire about socio-economic status and tooth brushing behavior, and oral hygiene status was measured by using Stallard et al. plaque index. One month after implementation, oral hygiene status was measured again. Implementation in the experimental group was to teach school teachers how to brush their teeth, and how to teach tooth brushing, effectively. All three groups received tooth brushing sets for the children. The results showed that baseline oral hygiene status was not significantly different between the experimental group and the same school control group, but a significant association was found between these two groups and the different school control groups, in the following areas buccal side of upper right first molar, labial side of upper right central incisor and lower left central incisor. After implementation, oral hygiene status of the experimental group was significantly improved nearly all surfaces, except buccal side of upper left first molar. The same school control group significantly improved on some surfaces; buccal side of upper right first molar, labial side of upper right central incisor and lower left central incisor. The different school control group had a higher score than baseline on lingual side of lower left and right first molar and labial side of lower left central incisor. Among the 3 groups, oral hygiene of the experimental group and the same school control group was significantly better than the different school control group. When compare in the same school, the oral hygiene of experimental group was significantly better than the control group on labial side of lower left central incisor. Therefore; the oral hygiene status of primary school children was improved by this education technique, however, the environment had the effect to change the oral hygiene of children.