The aim of this study was to compare the effect of different disinfection/sterilization methods on the mechanical properties of orthodontic elastomeric chain in terms of initial tensile force. A total of 150 elastomeric chains from three manufacturers were divided into 5 groups and tested with 70% ethanol, 0.12% chlorhexidine, 2.5% umonium^38® and autoclave method. Unexposed chains were also tested as control group. Initial tensile load was determined by stretching the elastomeric chain using a universal testing machine. Significant differences in tensile load were observed among different manufacturers (p<0.05). Compared to unexposed chains, tensile force of elastomeric chain in autoclave group significantly decreased (p<0.05). However, there was no significant differences after immersion in disinfection solutions including 70% ethanol and 2.5% umonium^38®. In conclusion, sterilization by autoclave affects the mechanical properties of elastomeric chain. Short exposure of elastomeric chain to disinfectant solutions before use intraorally is recommended in orthodontic clinic.